|
| 1 | +# RFC-6: Multiscale |
| 2 | + |
| 3 | +Turn the `multiscales` array into a single `multiscale` object. |
| 4 | + |
| 5 | + |
| 6 | +## Status |
| 7 | + |
| 8 | +This RFC is currently open for reviews (R1). |
| 9 | + |
| 10 | +```{list-table} Record |
| 11 | +:widths: 8, 20, 20, 20, 15, 10 |
| 12 | +:header-rows: 1 |
| 13 | +:stub-columns: 1 |
| 14 | +
|
| 15 | +* - Role |
| 16 | + - Name |
| 17 | + - GitHub Handle |
| 18 | + - Institution |
| 19 | + - Date |
| 20 | + - Status |
| 21 | +* - Author |
| 22 | + - Norman Rzepka |
| 23 | + - @normanrz |
| 24 | + - scalable minds |
| 25 | + - 2024-12-03 |
| 26 | + - |
| 27 | +* - Author |
| 28 | + - David Stansby |
| 29 | + - @dstansby |
| 30 | + - University College London |
| 31 | + - 2024-12-03 |
| 32 | + - |
| 33 | +* - Endorser |
| 34 | + - Davis Bennett |
| 35 | + - @d-v-b |
| 36 | + - |
| 37 | + - 2024-12-12 |
| 38 | + - |
| 39 | +* - Endorser |
| 40 | + - Will Moore |
| 41 | + - @will-moore |
| 42 | + - OME, University of Dundee |
| 43 | + - 2024-12-12 |
| 44 | + - |
| 45 | +* - Endorser |
| 46 | + - Lachlan Deakin |
| 47 | + - @LDeakin |
| 48 | + - Australian National University |
| 49 | + - 2024-12-17 |
| 50 | + - |
| 51 | +* - Endorser |
| 52 | + - Joel Lüthi |
| 53 | + - @jluethi |
| 54 | + - BioVisionCenter, University of Zurich |
| 55 | + - 2024-12-18 |
| 56 | + - |
| 57 | +* - Endorser |
| 58 | + - Eric Perlman |
| 59 | + - @perlman |
| 60 | + - |
| 61 | + - 2024-12-18 |
| 62 | + - |
| 63 | +``` |
| 64 | + |
| 65 | +## Overview |
| 66 | + |
| 67 | +This RFC proposes to change the `multiscales` array into a single `multiscale` object. |
| 68 | + |
| 69 | +## Background |
| 70 | + |
| 71 | +The current spec of OME-Zarr (version 0.5) defines that the metadata for a multiscale is stored in a `multiscales` array. |
| 72 | + |
| 73 | +However, there seem to be only very few OME-Zarr images with mutltiple multiscales in the wild. There is one example from IDR: [4995115.zarr](https://ome.github.io/ome-ngff-validator/?source=https://uk1s3.embassy.ebi.ac.uk/idr/zarr/v0.4/idr0050A/4995115.zarr) |
| 74 | + |
| 75 | +Additionally, most visualization and processing tools today simply process the first multiscale object in the `multiscales` array, ignoring any subsequent entries. Here is a selection of tools that only load the first multiscale object: |
| 76 | + |
| 77 | +- [neuroglancer](https://github.com/google/neuroglancer/blob/master/src/datasource/zarr/ome.ts#L265-L310) |
| 78 | +- [vizarr](https://github.com/hms-dbmi/vizarr/blob/main/src/utils.ts#L88) |
| 79 | +- [itk-vtk](https://github.com/Kitware/itk-vtk-viewer/blob/master/src/IO/ZarrMultiscaleSpatialImage.js#L173) |
| 80 | +- [OMERO](https://github.com/ome/ZarrReader/issues/44) |
| 81 | + |
| 82 | +The current spec seems to acknowledge that this is to be expected to some degree in the following excerpt: |
| 83 | + |
| 84 | +> If only one multiscale is provided, use it. Otherwise, the user can choose by name, using the first multiscale as a fallback: |
| 85 | +> |
| 86 | +> ```python |
| 87 | +> datasets = [] |
| 88 | +> for named in multiscales: |
| 89 | +> if named["name"] == "3D": |
| 90 | +> datasets = [x["path"] for x in named["datasets"]] |
| 91 | +> break |
| 92 | +> if not datasets: |
| 93 | +> # Use the first by default. Or perhaps choose based on chunk size. |
| 94 | +> datasets = [x["path"] for x in multiscales[0]["datasets"]] |
| 95 | +> ``` |
| 96 | +
|
| 97 | +
|
| 98 | +This RFC aims to codify what already seems to be common practice by moving from a multiscales array to a single multiscale object. This will reduce complexity for implementations. |
| 99 | +
|
| 100 | +## Proposal |
| 101 | +
|
| 102 | +The OME-Zarr metadata in a `zarr.json` file of a multiscale MUST contain a single `multiscale` object. This replaces the current `multiscales` array. |
| 103 | +
|
| 104 | +Adapted example from the current spec: |
| 105 | +```json |
| 106 | +{ |
| 107 | + "zarr_format": 3, |
| 108 | + "node_type": "group", |
| 109 | + "attributes": { |
| 110 | + "ome": { |
| 111 | + "version": "0.5", |
| 112 | + "multiscale": { |
| 113 | + "name": "example", |
| 114 | + "axes": [ |
| 115 | + { "name": "t", "type": "time", "unit": "millisecond" }, |
| 116 | + { "name": "c", "type": "channel" }, |
| 117 | + { "name": "z", "type": "space", "unit": "micrometer" }, |
| 118 | + { "name": "y", "type": "space", "unit": "micrometer" }, |
| 119 | + { "name": "x", "type": "space", "unit": "micrometer" } |
| 120 | + ], |
| 121 | + "datasets": [ |
| 122 | + { |
| 123 | + "path": "0", |
| 124 | + "coordinateTransformations": [ |
| 125 | + { |
| 126 | + // the voxel size for the first scale level (0.5 micrometer) |
| 127 | + "type": "scale", |
| 128 | + "scale": [1.0, 1.0, 0.5, 0.5, 0.5] |
| 129 | + } |
| 130 | + ] |
| 131 | + }, |
| 132 | + { |
| 133 | + "path": "1", |
| 134 | + "coordinateTransformations": [ |
| 135 | + { |
| 136 | + // the voxel size for the second scale level (downscaled by a factor of 2 -> 1 micrometer) |
| 137 | + "type": "scale", |
| 138 | + "scale": [1.0, 1.0, 1.0, 1.0, 1.0] |
| 139 | + } |
| 140 | + ] |
| 141 | + } |
| 142 | + ], |
| 143 | + "coordinateTransformations": [ |
| 144 | + { |
| 145 | + // the time unit (0.1 milliseconds), which is the same for each scale level |
| 146 | + "type": "scale", |
| 147 | + "scale": [0.1, 1.0, 1.0, 1.0, 1.0] |
| 148 | + } |
| 149 | + ] |
| 150 | + } |
| 151 | + } |
| 152 | + } |
| 153 | +} |
| 154 | +``` |
| 155 | +
|
| 156 | +For data that needs to have multiple multiscales, it is encouraged to store them in separate OME-Zarr datasets with their own OME-Zarr metadata. |
| 157 | +
|
| 158 | +
|
| 159 | +## Requirements |
| 160 | +
|
| 161 | +The key words "MUST", "MUST NOT", "REQUIRED", "SHALL", "SHALL NOT", "SHOULD", "SHOULD NOT", "RECOMMENDED", "MAY", and "OPTIONAL" in this document are to be interpreted as described in [IETF RFC 2119](https://tools.ietf.org/html/rfc2119) |
| 162 | +
|
| 163 | +
|
| 164 | +## Stakeholders |
| 165 | +
|
| 166 | +The main stakeholders for this RFC are OME-Zarr tool developers and existing OME-Zarr image providers. Developers will have to update their implementations to account for the breaking change. Because this change is not backwards compatible, it will require a change to existing OME-Zarr images to make them compatible with this RFC. |
| 167 | +
|
| 168 | +### Socialization |
| 169 | +
|
| 170 | +* OME-NGFF hackathon Zurich 2024 |
| 171 | +* [Github issue](https://github.com/ome/ngff/issues/205) |
| 172 | +
|
| 173 | +## Implementation |
| 174 | +
|
| 175 | +Many visualization and processing tools already expect only a single multiscale. |
| 176 | +This proposal will reduce complexity for implementations. |
| 177 | +
|
| 178 | +Examples of implementations that only work with a single multiscale: |
| 179 | +- [neuroglancer](https://github.com/google/neuroglancer/blob/master/src/datasource/zarr/ome.ts#L265-L310) |
| 180 | +- [vizarr](https://github.com/hms-dbmi/vizarr/blob/main/src/utils.ts#L88) |
| 181 | +- [itk-vtk](https://github.com/Kitware/itk-vtk-viewer/blob/master/src/IO/ZarrMultiscaleSpatialImage.js#L173) |
| 182 | +- [OMERO](https://github.com/ome/ZarrReader/issues/44) |
| 183 | +
|
| 184 | +## Drawbacks, risks, alternatives, and unknowns |
| 185 | +
|
| 186 | +This is a breaking change with the typical drawbacks of breaking changes. |
| 187 | +
|
| 188 | +## Compatibility |
| 189 | +
|
| 190 | +This proposal is not backwards compatible and should be released in a new version of OME-Zarr. |
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