diff --git a/beacon-chain/node/BUILD.bazel b/beacon-chain/node/BUILD.bazel
index 0d0f562d7b00..470f7919ac8f 100644
--- a/beacon-chain/node/BUILD.bazel
+++ b/beacon-chain/node/BUILD.bazel
@@ -40,6 +40,7 @@ go_library(
         "@com_github_pkg_errors//:go_default_library",
         "@com_github_sirupsen_logrus//:go_default_library",
         "@com_github_urfave_cli_v2//:go_default_library",
+        "@in_gopkg_yaml_v2//:go_default_library",
     ],
 )
 
diff --git a/beacon-chain/node/node.go b/beacon-chain/node/node.go
index 9fa5602ba2e3..12bbf72dfcc7 100644
--- a/beacon-chain/node/node.go
+++ b/beacon-chain/node/node.go
@@ -48,6 +48,7 @@ import (
 	"github.com/prysmaticlabs/prysm/shared/version"
 	"github.com/sirupsen/logrus"
 	"github.com/urfave/cli/v2"
+	"gopkg.in/yaml.v2"
 )
 
 var log = logrus.WithField("prefix", "node")
@@ -114,7 +115,7 @@ func NewBeaconNode(cliCtx *cli.Context) (*BeaconNode, error) {
 	}
 	if cliCtx.IsSet(cmd.BootstrapNode.Name) {
 		c := params.BeaconNetworkConfig()
-		c.BootstrapNodes = strings.Split(cliCtx.String(cmd.BootstrapNode.Name), ",")
+		c.BootstrapNodes = cliCtx.StringSlice(cmd.BootstrapNode.Name)
 		params.OverrideBeaconNetworkConfig(c)
 	}
 	if cliCtx.IsSet(flags.ContractDeploymentBlock.Name) {
@@ -315,16 +316,32 @@ func (b *BeaconNode) startStateGen() {
 	b.stateGen = stategen.New(b.db, b.stateSummaryCache)
 }
 
+func readbootNodes(fileName string) ([]string, error) {
+	fileContent, err := ioutil.ReadFile(fileName)
+	if err != nil {
+		return nil, err
+	}
+	listNodes := make([]string, 0)
+	err = yaml.Unmarshal(fileContent, &listNodes)
+	if err != nil {
+		return nil, err
+	}
+	return listNodes, nil
+}
+
 func (b *BeaconNode) registerP2P(cliCtx *cli.Context) error {
-	// Bootnode ENR may be a filepath to an ENR file.
-	bootnodeAddrs := params.BeaconNetworkConfig().BootstrapNodes
-	for i, addr := range bootnodeAddrs {
-		if filepath.Ext(addr) == ".enr" {
-			b, err := ioutil.ReadFile(addr)
+	// Bootnode ENR may be a filepath to a YAML file
+	bootnodesTemp := params.BeaconNetworkConfig().BootstrapNodes //actual CLI values
+	bootnodeAddrs := make([]string, 0)                           //dest of final list of nodes
+	for _, addr := range bootnodesTemp {
+		if filepath.Ext(addr) == ".yaml" {
+			fileNodes, err := readbootNodes(addr)
 			if err != nil {
 				return err
 			}
-			bootnodeAddrs[i] = string(b)
+			bootnodeAddrs = append(bootnodeAddrs, fileNodes...)
+		} else {
+			bootnodeAddrs = append(bootnodeAddrs, addr)
 		}
 	}
 
diff --git a/beacon-chain/node/node_test.go b/beacon-chain/node/node_test.go
index 3569bcd6094b..98456da1e259 100644
--- a/beacon-chain/node/node_test.go
+++ b/beacon-chain/node/node_test.go
@@ -3,6 +3,7 @@ package node
 import (
 	"flag"
 	"fmt"
+	"io/ioutil"
 	"os"
 	"testing"
 
@@ -47,3 +48,34 @@ func TestNodeClose_OK(t *testing.T) {
 		t.Log(err)
 	}
 }
+
+func TestBootStrapNodeFile(t *testing.T) {
+	file, err := ioutil.TempFile(testutil.TempDir(), "bootstrapFile")
+	if err != nil {
+		t.Fatalf("Error in TempFile call:  %v", err)
+	}
+	defer func() {
+		if err := os.Remove(file.Name()); err != nil {
+			t.Log(err)
+		}
+	}()
+
+	sampleNode0 := "- enr:-Ku4QMKVC_MowDsmEa20d5uGjrChI0h8_KsKXDmgVQbIbngZV0i" +
+		"dV6_RL7fEtZGo-kTNZ5o7_EJI_vCPJ6scrhwX0Z4Bh2F0dG5ldHOIAAAAAAAAAACEZXRoMpD" +
+		"1pf1CAAAAAP__________gmlkgnY0gmlwhBLf22SJc2VjcDI1NmsxoQJxCnE6v_x2ekgY_uo" +
+		"E1rtwzvGy40mq9eD66XfHPBWgIIN1ZHCCD6A"
+	sampleNode1 := "- enr:-TESTNODE2"
+	sampleNode2 := "- enr:-TESTNODE3"
+	err = ioutil.WriteFile(file.Name(), []byte(sampleNode0+"\n"+sampleNode1+"\n"+sampleNode2), 0644)
+	if err != nil {
+		t.Fatalf("Error in WriteFile call:  %v", err)
+	}
+	nodeList, err := readbootNodes(file.Name())
+	if err != nil {
+		t.Fatalf("Error in readbootNodes call:  %v", err)
+	}
+	if nodeList[0] != sampleNode0[2:] || nodeList[1] != sampleNode1[2:] || nodeList[2] != sampleNode2[2:] {
+		// nodeList's YAML parsing will have removed the leading "- "
+		t.Fatalf("TestBootStrapNodeFile failed.  Nodes do not match")
+	}
+}
diff --git a/shared/cmd/flags.go b/shared/cmd/flags.go
index 462f58be24ad..4a113b2ab339 100644
--- a/shared/cmd/flags.go
+++ b/shared/cmd/flags.go
@@ -80,10 +80,10 @@ var (
 		Usage: "Connect with this peer. This flag may be used multiple times.",
 	}
 	// BootstrapNode tells the beacon node which bootstrap node to connect to
-	BootstrapNode = &cli.StringFlag{
+	BootstrapNode = &cli.StringSliceFlag{
 		Name:  "bootstrap-node",
-		Usage: "The address of bootstrap node. Beacon node will connect for peer discovery via DHT.  Multiple nodes can be separated with a comma",
-		Value: "enr:-Ku4QMKVC_MowDsmEa20d5uGjrChI0h8_KsKXDmgVQbIbngZV0idV6_RL7fEtZGo-kTNZ5o7_EJI_vCPJ6scrhwX0Z4Bh2F0dG5ldHOIAAAAAAAAAACEZXRoMpD1pf1CAAAAAP__________gmlkgnY0gmlwhBLf22SJc2VjcDI1NmsxoQJxCnE6v_x2ekgY_uoE1rtwzvGy40mq9eD66XfHPBWgIIN1ZHCCD6A",
+		Usage: "The address of bootstrap node. Beacon node will connect for peer discovery via DHT.  Multiple nodes can be passed by using the flag multiple times but not comma-separated. You can also pass YAML files containing multiple nodes.",
+		Value: cli.NewStringSlice("enr:-Ku4QMKVC_MowDsmEa20d5uGjrChI0h8_KsKXDmgVQbIbngZV0idV6_RL7fEtZGo-kTNZ5o7_EJI_vCPJ6scrhwX0Z4Bh2F0dG5ldHOIAAAAAAAAAACEZXRoMpD1pf1CAAAAAP__________gmlkgnY0gmlwhBLf22SJc2VjcDI1NmsxoQJxCnE6v_x2ekgY_uoE1rtwzvGy40mq9eD66XfHPBWgIIN1ZHCCD6A"),
 	}
 	// RelayNode tells the beacon node which relay node to connect to.
 	RelayNode = &cli.StringFlag{