diff --git a/docs/manuals/measurement/restless_measurements.rst b/docs/manuals/measurement/restless_measurements.rst
index 86f2143357..d42821fe94 100644
--- a/docs/manuals/measurement/restless_measurements.rst
+++ b/docs/manuals/measurement/restless_measurements.rst
@@ -52,7 +52,8 @@ In Qiskit Experiments, the experiments that support restless measurements
 have a special method :meth:`~.RestlessMixin.enable_restless` to set the restless run options
 and define the data processor that will process the measured data.
 If you are an experiment developer, you can add the :class:`.RestlessMixin`
-to your experiment class to add support for restless measurements.
+to your experiment class to add support for restless measurements. For correct method resolution order,
+:class:`.RestlessMixin` should be the first base class your class will inherit from.
 Here, we will show how to activate restless measurements using
 a fake backend and a rough DRAG experiment. Note however, that you will not
 observe any meaningful outcomes with fake backends since the circuit simulator
@@ -89,9 +90,11 @@ they use always starts with the qubits in the ground state.
 
 As you can see, a restless data processor is automatically chosen for the experiment. This
 data processor post-processes the restless measured shots according to the order in which
-they were acquired. Furthermore, the appropriate run options are also set. Note that
-these run options might be unique to IBM Quantum providers. Therefore, execute may fail
-on non-IBM Quantum providers if the required options are not supported.
+tthey were acquired. Furthermore, the appropriate run options are also set. Those run options would also
+override new run options that will be set afterward. To disable this override, one should set the
+option `restless` to `False`. Note that these run options might be unique to IBM Quantum providers.
+Therefore, execute may fail on non-IBM Quantum providers if the required options are not supported.
+
 
 After calling :meth:`~.RestlessMixin.enable_restless` the experiment is ready to be run
 in a restless mode. With a hardware backend, this would be done by calling the
diff --git a/qiskit_experiments/framework/base_experiment.py b/qiskit_experiments/framework/base_experiment.py
index 41240df41c..3768797646 100644
--- a/qiskit_experiments/framework/base_experiment.py
+++ b/qiskit_experiments/framework/base_experiment.py
@@ -451,7 +451,7 @@ def set_transpile_options(self, **fields):
     @classmethod
     def _default_run_options(cls) -> Options:
         """Default options values for the experiment :meth:`run` method."""
-        return Options(meas_level=MeasLevel.CLASSIFIED)
+        return Options(meas_level=MeasLevel.CLASSIFIED, restless=False)
 
     @property
     def run_options(self) -> Options:
diff --git a/qiskit_experiments/framework/restless_mixin.py b/qiskit_experiments/framework/restless_mixin.py
index 19d65c238f..3e24f15127 100644
--- a/qiskit_experiments/framework/restless_mixin.py
+++ b/qiskit_experiments/framework/restless_mixin.py
@@ -34,8 +34,10 @@ class RestlessMixin:
     This class defines the following methods:
 
     - :meth:`~.RestlessMixin.enable_restless`
+    - :meth:`~.RestlessMixin._enable_restless`
     - :meth:`~.RestlessMixin._get_restless_processor`
     - :meth:`~.RestlessMixin._t1_check`
+    - :meth:`~.RestlessMixin._finalize`
 
     A restless enabled experiment is an experiment that can be run in a restless
     measurement setting. In restless measurements, the qubit is not reset after
@@ -61,19 +63,28 @@ class makes it easy to determine if restless measurements are supported for a gi
 
     analysis: BaseAnalysis
     _default_run_options: Options()
+    run_options: Options()
     set_run_options: Callable
     _backend: Backend
     _physical_qubits: Sequence[int]
     _num_qubits: int
 
+    def __init__(self, *args, **kwargs):
+        super().__init__(*args, **kwargs)
+        # self._enable_restless = False
+        self.rep_delay = None
+        self.override_processor_by_restless = None
+        self.suppress_t1_error = None
+
     def enable_restless(
         self,
         rep_delay: Optional[float] = None,
         override_processor_by_restless: bool = True,
         suppress_t1_error: bool = False,
     ):
-        """Enables a restless experiment by setting the restless run options and the
-        restless data processor.
+        """
+        Set a flag to enable restless configuration when running the experiment. If the flag is set to
+        `True`, the program will run `_enable_restless` method in the `_finilized` step.
 
         Args:
             rep_delay: The repetition delay. This is the delay between a measurement
@@ -88,6 +99,26 @@ def enable_restless(
                 ``rep_delay`` is larger than the T1 times of the qubits. Instead, a warning will
                 be logged as restless measurements may have a large amount of noise.
 
+        """
+
+        self.set_run_options(restless=True)
+        if rep_delay:
+            self.rep_delay = rep_delay
+        if override_processor_by_restless:
+            self.override_processor_by_restless = override_processor_by_restless
+        if suppress_t1_error:
+            self.suppress_t1_error = suppress_t1_error
+
+        # Calling `_enable_restless()` so the run option will be visible to the user.
+        self._enable_restless()
+        # Setting override_processor_by_restless to false because
+        # the data processor was already configured.
+        self.override_processor_by_restless = False
+
+    def _enable_restless(self):
+        """Enables a restless experiment by setting the restless run options and the
+        restless data processor.
+
         Raises:
             DataProcessorError: If the attribute rep_delay_range is not defined for the backend.
             DataProcessorError: If a data processor has already been set but
@@ -98,10 +129,11 @@ def enable_restless(
                 T1 time of one of the physical qubits in the experiment and the flag
                 ``ignore_t1_check`` is False.
         """
+        LOG.debug("Enabling restless configuration. This will override current configuration.")
         try:
-            if not rep_delay:
+            if not self.rep_delay:
                 # BackendV1 only; BackendV2 does not support this
-                rep_delay = self._backend.configuration().rep_delay_range[0]
+                self.rep_delay = self._backend.configuration().rep_delay_range[0]
         except AttributeError as error:
             raise DataProcessorError(
                 "The restless experiment can not be enabled because "
@@ -110,15 +142,15 @@ def enable_restless(
             ) from error
 
         # Check the rep_delay compared to the T1 time.
-        if not self._t1_check(rep_delay):
+        if not self._t1_check():
             msg = (
-                f"The specified repetition delay {rep_delay} is equal to or greater "
+                f"The specified repetition delay {self.rep_delay} is equal to or greater "
                 f"than the T1 time of one of the physical qubits"
                 f"{self._physical_qubits} in the experiment. Consider choosing "
                 f"a smaller repetition delay for the restless experiment."
             )
 
-            if suppress_t1_error:
+            if self.suppress_t1_error:
                 LOG.warning(msg)
             else:
                 raise DataProcessorError(msg)
@@ -129,7 +161,7 @@ def enable_restless(
         meas_return = self._default_run_options().get("meas_return", MeasReturnType.SINGLE)
         if not self.analysis.options.get("data_processor", None):
             self.set_run_options(
-                rep_delay=rep_delay,
+                rep_delay=self.rep_delay,
                 init_qubits=False,
                 memory=True,
                 meas_level=meas_level,
@@ -146,9 +178,9 @@ def enable_restless(
                     "does not have the data_processor option."
                 )
         else:
-            if not override_processor_by_restless:
+            if not self.override_processor_by_restless:
                 self.set_run_options(
-                    rep_delay=rep_delay,
+                    rep_delay=self.rep_delay,
                     init_qubits=False,
                     memory=True,
                     meas_level=meas_level,
@@ -188,13 +220,9 @@ def _get_restless_processor(self, meas_level: int = MeasLevel.CLASSIFIED) -> Dat
                 ],
             )
 
-    def _t1_check(self, rep_delay: float) -> bool:
+    def _t1_check(self) -> bool:
         """Check that repetition delay < T1 of the physical qubits in the experiment.
 
-        Args:
-            rep_delay: The repetition delay. This is the delay between a measurement
-                    and the subsequent quantum circuit.
-
         Returns:
             True if the repetition delay is smaller than the qubit T1 times.
 
@@ -209,7 +237,7 @@ def _t1_check(self, rep_delay: float) -> bool:
                 for physical_qubit in self._physical_qubits
             ]
 
-            if all(rep_delay / t1_value < 1.0 for t1_value in t1_values):
+            if all(self.rep_delay / t1_value < 1.0 for t1_value in t1_values):
                 return True
         except AttributeError as error:
             raise DataProcessorError(
@@ -218,3 +246,9 @@ def _t1_check(self, rep_delay: float) -> bool:
             ) from error
 
         return False
+
+    def _finalize(self):
+        # Calling again to self._enable_restless() to override experiment option for restless experiment.
+        if self.run_options.get("restless", None):
+            self._enable_restless()
+        super()._finalize()
diff --git a/qiskit_experiments/library/characterization/drag.py b/qiskit_experiments/library/characterization/drag.py
index 6a338212e8..2c00edda7d 100644
--- a/qiskit_experiments/library/characterization/drag.py
+++ b/qiskit_experiments/library/characterization/drag.py
@@ -26,7 +26,7 @@
 from qiskit_experiments.library.characterization.analysis import DragCalAnalysis
 
 
-class RoughDrag(BaseExperiment, RestlessMixin):
+class RoughDrag(RestlessMixin, BaseExperiment):
     r"""An experiment that scans the DRAG parameter to find the optimal value.
 
     # section: overview
diff --git a/qiskit_experiments/library/characterization/fine_amplitude.py b/qiskit_experiments/library/characterization/fine_amplitude.py
index 69232e2e48..937bcc502a 100644
--- a/qiskit_experiments/library/characterization/fine_amplitude.py
+++ b/qiskit_experiments/library/characterization/fine_amplitude.py
@@ -25,7 +25,7 @@
 from qiskit_experiments.library.characterization.analysis import FineAmplitudeAnalysis
 
 
-class FineAmplitude(BaseExperiment, RestlessMixin):
+class FineAmplitude(RestlessMixin, BaseExperiment):
     r"""An experiment to determine the optimal pulse amplitude by amplifying gate errors.
 
     # section: overview
diff --git a/qiskit_experiments/library/characterization/fine_drag.py b/qiskit_experiments/library/characterization/fine_drag.py
index 24dafb060c..a1fca017ba 100644
--- a/qiskit_experiments/library/characterization/fine_drag.py
+++ b/qiskit_experiments/library/characterization/fine_drag.py
@@ -24,7 +24,7 @@
 from qiskit_experiments.curve_analysis.standard_analysis import ErrorAmplificationAnalysis
 
 
-class FineDrag(BaseExperiment, RestlessMixin):
+class FineDrag(RestlessMixin, BaseExperiment):
     r"""An experiment that performs fine characterizations of DRAG pulse coefficients.
 
     # section: overview
diff --git a/qiskit_experiments/library/characterization/rabi.py b/qiskit_experiments/library/characterization/rabi.py
index 3fb2a682d6..bc165f05f1 100644
--- a/qiskit_experiments/library/characterization/rabi.py
+++ b/qiskit_experiments/library/characterization/rabi.py
@@ -27,7 +27,7 @@
 from qiskit_experiments.curve_analysis import ParameterRepr, OscillationAnalysis
 
 
-class Rabi(BaseExperiment, RestlessMixin):
+class Rabi(RestlessMixin, BaseExperiment):
     r"""An experiment that scans a pulse amplitude to calibrate rotations on the :math:`|0\rangle`
     <-> :math:`|1\rangle` transition.
 
diff --git a/qiskit_experiments/library/characterization/ramsey_xy.py b/qiskit_experiments/library/characterization/ramsey_xy.py
index ccb1987481..22f0dbae19 100644
--- a/qiskit_experiments/library/characterization/ramsey_xy.py
+++ b/qiskit_experiments/library/characterization/ramsey_xy.py
@@ -23,7 +23,7 @@
 from qiskit_experiments.library.characterization.analysis import RamseyXYAnalysis
 
 
-class RamseyXY(BaseExperiment, RestlessMixin):
+class RamseyXY(RestlessMixin, BaseExperiment):
     r"""A sign-sensitive experiment to measure the frequency of a qubit.
 
     # section: overview
diff --git a/qiskit_experiments/library/randomized_benchmarking/standard_rb.py b/qiskit_experiments/library/randomized_benchmarking/standard_rb.py
index 9a7795155a..40c2ee43d6 100644
--- a/qiskit_experiments/library/randomized_benchmarking/standard_rb.py
+++ b/qiskit_experiments/library/randomized_benchmarking/standard_rb.py
@@ -53,7 +53,7 @@
 SequenceElementType = Union[Clifford, Integral, QuantumCircuit]
 
 
-class StandardRB(BaseExperiment, RestlessMixin):
+class StandardRB(RestlessMixin, BaseExperiment):
     """An experiment to characterize the error rate of a gate set on a device.
 
     # section: overview
diff --git a/releasenotes/notes/inforcing-restless-options-9a659248108c2533.yaml b/releasenotes/notes/inforcing-restless-options-9a659248108c2533.yaml
new file mode 100644
index 0000000000..5218f8dcd2
--- /dev/null
+++ b/releasenotes/notes/inforcing-restless-options-9a659248108c2533.yaml
@@ -0,0 +1,7 @@
+---
+features:
+  - |
+    `RestlessMixin` class will now override the experiment run option at `_finilize()` method.
+    `RestlessMixin` class should be the first base class that an experiment inherits from for correct
+    MRO (Method Resolution Order). To cancel this override, set `restless` to False in the experiment
+    options.
diff --git a/test/data_processing/test_restless_experiment.py b/test/data_processing/test_restless_experiment.py
index 7a02e3768e..87c21c4979 100644
--- a/test/data_processing/test_restless_experiment.py
+++ b/test/data_processing/test_restless_experiment.py
@@ -37,9 +37,11 @@ def test_enable_restless(self):
 
         error = -np.pi * 0.01
         backend = MockRestlessFineAmp(error, np.pi, "x")
+        amp_exp = FineXAmplitude([0], backend)
 
         with self.assertRaises(DataProcessorError):
-            FineXAmplitude([0], backend).enable_restless(rep_delay=2.0)
+            amp_exp.enable_restless(rep_delay=2.0)
+            amp_exp.run()
 
         amp_exp = FineXAmplitude([0], backend)
         amp_exp.enable_restless(rep_delay=2.0, suppress_t1_error=True)
diff --git a/test/fake_experiment.py b/test/fake_experiment.py
index 80236e8e74..d992dd3bc1 100644
--- a/test/fake_experiment.py
+++ b/test/fake_experiment.py
@@ -69,13 +69,15 @@ def _default_experiment_options(cls) -> Options:
         options.dummyoption = None
         return options
 
-    def __init__(self, physical_qubits=None, backend=None, experiment_type=None):
+    def __init__(self, physical_qubits=None, backend=None, analysis=None, experiment_type=None):
         """Initialise the fake experiment."""
         if physical_qubits is None:
             physical_qubits = [0]
+        if analysis is None:
+            analysis = FakeAnalysis()
         super().__init__(
             physical_qubits,
-            analysis=FakeAnalysis(),
+            analysis=analysis,
             backend=backend,
             experiment_type=experiment_type,
         )
diff --git a/test/framework/test_composite.py b/test/framework/test_composite.py
index c1667a3f60..c43135014f 100644
--- a/test/framework/test_composite.py
+++ b/test/framework/test_composite.py
@@ -65,7 +65,7 @@ def test_parallel_options(self):
         par_exp = ParallelExperiment([exp0, exp2], flatten_results=False)
 
         self.assertEqual(par_exp.experiment_options, par_exp._default_experiment_options())
-        self.assertEqual(par_exp.run_options, Options(meas_level=2))
+        self.assertEqual(par_exp.run_options, Options(meas_level=2, restless=False))
         self.assertEqual(par_exp.transpile_options, Options(optimization_level=0))
         self.assertEqual(par_exp.analysis.options, par_exp.analysis._default_options())
 
diff --git a/test/framework/test_framework.py b/test/framework/test_framework.py
index 1a2ae395d3..904b6db793 100644
--- a/test/framework/test_framework.py
+++ b/test/framework/test_framework.py
@@ -22,7 +22,12 @@
 from qiskit import QuantumCircuit
 from qiskit.providers.jobstatus import JobStatus
 from qiskit.exceptions import QiskitError
+from qiskit.qobj.utils import MeasLevel
+from qiskit.result import Result
+
+
 from qiskit_ibm_runtime.fake_provider import FakeVigoV2
+import qiskit_experiments.data_processing as dp
 
 from qiskit_experiments.database_service import Qubit
 from qiskit_experiments.exceptions import AnalysisError
@@ -33,6 +38,8 @@
     BaseAnalysis,
     AnalysisResultData,
     AnalysisStatus,
+    RestlessMixin,
+    Options,
 )
 from qiskit_experiments.test.fake_backend import FakeBackend
 from qiskit_experiments.test.utils import FakeJob
@@ -426,3 +433,79 @@ def circuits(self):
         self.assertEqual(exp2.experiment_type, "MyExp")
         exp2.experiment_type = "suieee"
         self.assertEqual(exp2.experiment_type, "suieee")
+
+    def test_restless_experiment_options(self):
+        """Test override of experiment option in restless experiment."""
+        # pylint: disable-next=redefined-outer-name, reimported
+        from qiskit_experiments.test.utils import FakeJob
+        import uuid
+
+        class FakeRestlessBackend(FakeVigoV2):
+            """Fake backend for restless experiment"""
+
+            def run(self, run_input, **options):
+                result = {
+                    "backend_name": "fake_backend",
+                    "backend_version": "0",
+                    "qobj_id": uuid.uuid4().hex,
+                    "job_id": uuid.uuid4().hex,
+                    "success": True,
+                    "results": [],
+                }
+                return FakeJob(backend=self, result=Result.from_dict(result))
+
+        class FakeRestlessAnalysis(FakeAnalysis):
+            """Fake analysis class for fake restless experiment. We need this for the data processor."""
+
+            @classmethod
+            def _default_options(cls) -> Options:
+                """Default analysis options."""
+                options = super()._default_options()
+
+                options.update_options(
+                    data_processor=dp.DataProcessor("counts", [dp.Probability("1")]),
+                    stark_coefficients="latest",
+                    x_key="xval",
+                )
+                return options
+
+        class FakeRestlessExperiment(RestlessMixin, FakeExperiment):
+            """Fake restless experiment for testing."""
+
+            def __init__(
+                self, physical_qubits=None, backend=None, experiment_type="FakeRestlessExperiment"
+            ):
+                """Initialise the fake experiment."""
+                if physical_qubits is None:
+                    physical_qubits = [0]
+                super().__init__(
+                    physical_qubits,
+                    analysis=FakeRestlessAnalysis(),
+                    backend=backend,
+                    experiment_type=experiment_type,
+                )
+
+            @classmethod
+            def _default_run_options(cls) -> Options:
+                """Default option values for the experiment :meth:`run` method."""
+                options = super()._default_run_options()
+
+                options.meas_level = MeasLevel.KERNELED
+                options.meas_return = "single"
+
+                return options
+
+        backend = FakeRestlessBackend()
+        exp = FakeRestlessExperiment(backend=backend)
+
+        exp.enable_restless(
+            override_processor_by_restless=False, rep_delay=250, suppress_t1_error=True
+        )
+        exp.run_options.meas_level = MeasLevel.CLASSIFIED
+        self.assertNotEqual(exp.run_options.get("meas_level"), MeasLevel.KERNELED)
+
+        # `run()` method makes a copy of the experiment if run option is passed through it, and we cannot
+        # access it for testing.
+        # pylint: disable-next=unused-variable
+        expdata = exp.run()
+        self.assertEqual(exp.run_options.get("meas_level"), MeasLevel.KERNELED)